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   FACILITIES & EQUIPMENT
  equipment  
equip_side The HWI laboratories are fully equipped for conducting research in structural biology.  The resources available include the following:

Protein Production and Purification Laboratories: Shared laboratory facilities are:

  • two Applied BioSystems GeneAmp PCR systems,
  • nine Beckman-Coulter centrifuges (one Allegra 6KR, four Avanti J20, two L855 ultracentrifuges, one Optima MAX-E tabletop ultracentrifuge, and one J2-21M Induction Drive),
  • two Allegra X-22R and two Allegra X-12R tabletop centrifuges,
  • a Dupont Sorvall RC3C centrifuge,
  • two Agilent 8453 UV/Vis Spectrophotometers with multi-cell transport and Biochemical Analysis software packages,
  • four walk-in cold-rooms (~250 ft2 equipped with laboratory benches and chromatography equipment),
  • one Packard Liquid Scintillation Counter,
  • one Waters High Pressure Liquid Chromatography system with in-line radioactive detector,
  • three Pharmacia AKTA 10 Purifier systems,
  • five Pharmacia AKTA FPLC systems (all completely equipped with affinity and gel filtration columns and housed at 4°C), and
  • four Pharmacia AKTA Prime FPLC systems

 

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Standard items of laboratory equipment (balances, water baths, pH meters, etc…) are available.  There are significant eukaryotic cell growth facilities to produce proteins using yeast (Pichia) and Baculovirus expression systems.  These systems include dedicated tissue culture rooms with biosafety hoods, eight incubators, fourteen shakers, and the necessary glassware for cell growth.  A Microfluidics Corp. M-110EH Microfluidizer and three sonicators are available for cell disruption purposes. Additionally, a 10L New Brunswick BIOFLO 3000 Bioreactor/Fermentor is available for large-scale growths.

Crystal Growth Laboratory: HWI is home to a high-throughput screening laboratory for determining initial crystallization conditions.  This facility, under the direction of Joseph Luft, is a unique resource that provides rapid screening of 1536 conditions using the microbatch-under-oil crystallization technique. Two Robbins Scientific Tango liquid-handling systems are employed.  Two custom constructed reader tables digitally record images of these experiments.  This table is coupled with a dual-processor primary NFS server with failover server, a primary 0.5 terabyte RAID 5 subsystem, and a secondary 1 terabyte RAID 5 subsystem that takes snapshots of the primary system every four hours.  A tape library system (1.8 TB capacity), optical disc drives (CD and DVD), and an ftp server with a 0.5 terabyte RAID5 subsystem are integrated with a database for data distribution and storage.  Experiments are monitored using digital recordings of the results.  Crystallization experiments are stored in one of five temperature-controlled incubators (Precision Scientific Model 815). Sample homogeneity can be determined with both dynamic and static light scattering techniques (Protein Solutions Dynapro temperature-controlled dynamic light scattering instrument and a Wyatt Technology Corporation mini DAWN triple-angle light scattering detector. Specific physical and chemical properties of solutions can be measured with a Brookfield programmable DV-II viscometer, a Kruss K-11 tensiometer, and a Wescor 5520 Vapro Vapor Pressure Osmometer.  Full laboratory facilities are available for optimization of crystallization experiments.  Six stereomicroscopes are available, two of which are equipped with digital cameras.

X-ray Diffraction Facilities: X-Ray diffraction facilities include two area detectors.  An RU-H3RHB 18 kW rotating anode generator (Cu target) is equipped with MSC/Osmic Max-Flux confocal multilayer mirrors, a 40° 2q stage, an R-AXIS IV imaging plate system, and an X-Stream cryogenic system.  An R–AXIS IIc is installed on an RU-200 12 kW generator.  This system also includes a 2q table and an X-Stream cryogenic device.  It is equipped with a monochromator, and Mo, Cu and Cr targets are available.  The protein diffraction facilities also include a Cryo-Xe-Siter for the production of frozen Xe heavy-atom derivatives.  Also available is a Nonius CAD4 diffractometer on a Nonius 590 generator (Mo or Cu X-ray tubes) equipped with a conventional low-temperature device.

Computational Facilities: The computational resources of HWI are anchored by a 89-node Apple Xserve cluster.  Each node consists of dual 2.3 GHz processors with 2 GB of memory.  The local computational network is connected to the outside world via NYSERNet through a gigabit link to the University at Buffalo.

700 Ellicott Street Buffalo, New York 14203-1102 Tel: 716 898 8600 Fax: 716 898 8660